[Elimination of copper and zinc from industrial wastes by mutated bacteria]

Today, toxic effluents have created ecological and health problems in and around the industrial cities resulting in death of nearby living organisms. The aim of this research was to increase the elimination of copper and zinc from copper factory effluents in Kerman/Iran through mutation inducing in metal-resistant bacteria by using Acriflavine, Acridine orange and Ethidium bromide. A total of 20 strains of Pseudomonas spp. were isolated from water and soil of the factory and subjected to microbiological identification. Maximum Inhibitory Concentration [MIC] to Cu and Zn were determined by agar dilution method. Those strains with the highest MIC to these metals [5mM] were subjected to 400-3200mg/L of the above mutagenic agents. After determination of MIC those colonies which were capable to grow on 20mM copper were selected for atomic absorption spectroscopy. According to the atomic absorption spectroscopy of dried biomass obtained from resistant strains after exposure to mutagenic agents, strains 6,7,8,9,10,13 and 16 showed the highest accumulation of CU and Zn [10mM for Cu and 20mM for Zn]. Strain 13 had the highest absorption of Cu [0.35%/mg biomass] and strain 10 showed the highest accumulation of Zn [0.33%/mg biomass]. Elimination of heavy metals by artificially mutated bacteria can be suggested as a cost effective solution to this environmental health issue

Application of metal resistant bacteria by mutational enhancment technique for bioremediation of copper and zinc from industrial wastes

In this research, using mutation in the metal resistant bacteria, the bioremediation of the copper and zinc from copper factory effluents was investigated. Wastewater effluents from flocculation and rolling mill sections of a factory in the city of Kerman were collected and used for further experiments. 20 strains of Pseudomonas spp. were isolated from soil and effluents surrounding factory and identified by microbiological methods. Minimum inhibitory concentrations for copper [Cu] and zinc [Zn] were determined by agar dilution method. Those strains that exhibited highest minimum inhibitory concentrations values to the metals [5mM] were subjected to 400-3200 mg/L concentrations of the three mutagenic agents, acriflavine, acridine orange and ethidium bromide. After determination of subinhibitory concentrations, the minimum inhibitory concentrations values for copper and zinc metal ions were again determined, which showed more than 10 fold increase in minimum inhibitory concentrations value [10 mM for Cu and 20 mM for Zn] with P